Abstract: Objective To investigate the disinfection quality and provide scientific basis for the prevention and control of infectious diseases in nurseries and kindergartens in Changning District of Shanghai. Methods On-site investigation and sampling examination were used during the continuous monitoring of the effect of disinfection in nurseries and kindergartens in Changning District of Shanghai. Results The eligible rates of disinfection quality during 2010-2014 in the nurseries and kindergartens of Changning District in Shanghai were 98.07%,98.93%,99.09%,97.11% and 98.10% successively. The eligible rates of indoor air, surfaces of environmental objects,hands of staff, eating utensils,disinfection solution in use were 99.74%,99.67%,93.24%,99.06% and 98.09% respectively. Conclusion The sanitary disinfection quality in nurseries and kindergartens of Changning in Shanghai is satisfactory as a whole, however,there is still weakness in the disinfection quality of the hands of staff, disinfection solution in use and eating utensils.