Abstract: Objective To investigate Helicobacter Pyloris (Hp) infection in adolescents and prevalence of Hp resistance to commonly used antibiotics. Methods A series of 216 cases in our hospital were enrolled between March 1, 2008 and May 31, 2011. The research group did gastroscopy and grastric mucosal samples were taken for culture and isolation of Hp strains. And then the resistance rate to the antibiotics was tested by Kirby-Baner method. Results A total of 71 Hp strains were cultured and isolated from 216 adolescent patients and the positiveness rate was found to be 32.87%. In the group aged 6 years to 10, the positiveness rate was 17.86%(15/84),in the group aged 10 years to 14, 30.95%(13/42) and in the group aged 14 years to 18, 48.78%(43/90). There was markedly statistical difference in Hp infection rate between the three groups. Of the 71 Hp strains isolated from adolescent, the antibiotics resistance rate to Clarithromycin, Amoxicillin, Metronidazole, Fruranzolidone, Gentamicin and Levofloxacin was respectively 8.45%(6/71), 22.54%(16/71), 94.37%(67/71), 16.90%(12/71), 0% (0/71) and 0%(0/71). Conclusion Hp culture positiveness rate is increasing with the age rising, the prevalence of Hp resistance rate in adolescents to Metronidazole and Amoxicillin are relatively higher, as compared to the resistance rate to Clarithromycin and Fruranzolidone. There is no Hp strain found to be resistant to Gentamicin and Levofloxacin.