Abstract: Objective To set up an onsite protocol for abrupt emergencies of public health events, with both the sensitivity and accuracy of the lab-on-chip(microfluidic chip), for the detection of food borne pathogenic bacterium including Vibrio cholera, Salmonella, Vibrio parahaemolyticus and Shigellaare exanimated.Methods By comparison of the results from the chips and fluorescence quantitative PCR were analyzed the specificity, sensitivity, accuracy and repeatability of the Lab-on-chip.Results Acceptable specificity, accuracy and repeatability of the chips had been well achieved, the detection limit of the chips for the Vibrio cholera, Salmonella, Vibrio parahaemolyticus and Shigella was 5×10³~10³ DNA Copies/mL.Conclusion Lab-on-chip is believed to be a fast, convenient, efficient tool with acceptable accuracy for public health emergencies.