Abstract: Objective To investigate HPV infection and its typing situation in cervical disease patients in a hospital by flow fluorescence hybridization for detection of human papillomavirus (HPV). Methods From January to December 2013, a total of 4 041 cervical disease patients were as research objects. The cervical exfoliated cell specimens were collected and HPV genotypes were detected by flow fluorescence hybridization, while HPV infection rate, genetic subtypes, infection type and age distribution were analyzed. Results HPV infection was found in 744 cases(18.41%), in which 603 cases(14.92%) were single subtype and 141 cases(3.49%) multiple subtypes of infection. All 26 kinds of HPV subtypes available to detection were detected. The common subtypes were HPV-16, 52,58,39,18,61,59,6 etc., in which HPV-61,6,11 subtypes were with low-risk and HPV-16,52,58 subtypes with high-risk. More common type of infection was high-risk subtype, in which single high-risk subtype infection was 13.07%(528/4 041) and multiple high-risk subtypes infection 2.15%(87/4 041).The highest rate of HPV infection(22.22%) was found in the women 31 to 40 years of age, and the lowest(13.99%) in those above 50 years.In all age groups, the high-risk subtype infection was found with the highest detection rate. Conclusion Patients with cervical disease are found to have higher HPV infection rate with wider distribution genotype. Flow fluorescence hybridization proves a fast and effective method to detect HPV infection, and HPV genotyping has significance for prevention and treatment of cervical disease.