Abstract: Objective To investigate the implementation effect of health education path in neonatal disease screening, then promoting popularization and development of neonatal disease screening. Methods The health education path was worked out for neonatal disease screening. A total of 986 cases of single birth mothers and their newborns were as the observation group, in which the path of health education was implemented for them. And 1 052 cases of single birth mothers and their newborns were as the control group, in which the traditional method of health education was done. Comparison was made between the two groups in neonatal disease screening rate, positive recall rate, degree of parturient about understanding and mastering the knowledge of neonatal disease screening, parturient satisfaction and parental satisfaction. Results The neonatal disease screening rate, positive recall rate, degree of parturient about understanding and mastering the knowledge of neonatal disease screening, parturient satisfaction and parental satisfaction were all better in the observation group than those in the control group(P<0.05).Conclusion The health education path for neonatal disease screening can effectively promote its development and popularization.