Abstract: Objective To examine the prevalence of HIV and HCV infections as well as utilization of related health services among drug users in HIV-sentinel surveillance sites in Dehong Prefecture of Yunnan Province. Methods Drug users in four HIV sentinel surveillance sites in Dehong Prefecture during 2011-2013 were tested for HIV and HCV infections and were administered a questionnaire according to the National Protocol for HIV Sentinel Surveillance. Results A total of 4 878 person-times were monitored from 2011 to 2013. The prevalence of HIV and HCV infections was 11.6% and 20.5% for the 1 597 drug users in 2011, 9.6% and 23.9% for the 1 602 drug users in 2012, and 10.8% and 26.1% for the 1 679 drug users in 2013, respectively. Trend difference was observed for HCV prevalence (χ²=14.49, P=0.000) but not for HIV prevalence (χ²=0.52,P=0.471) in the three years. Utilization of HIV-related health services varied significantly in the three years. From 2011 to 2013, the proportion of receiving service of peer education was 24.4%, 56.4% and 51.9%; the proportion of receiving service of condom promotion and voluntary HIV counseling and testing was 44.3%, 69.0% and 60.3%; and the proportion of participating in service of community-based methadone maintenance treatment and/or needle exchange programs was 14.2%, 2.9% and 2.3%, respectively. Conclusion Drug users in HIV-sentinel surveillance sites in Dehong Prefecture have high prevalence of HIV and HCV infections but low utilization of HIV-related health education and behavioral interventions. Efforts are needed to promote health education and behavioral interventions targeting drug users.