Abstract: Objective To investigate the influences and effects on hygienic inspection management in public places after the implementation of the Regulations on Hygienic Management in Public Places(hereinafter referred to as Regulations). Methods Data of hygienic management and administrative penalty in public places of Hongkou District from 2009 to 2013 were analyzed and investigated. Furthermore, questionnaires and individual interviews were surveyed among 10% of proprietors and 250 employees in public places of Hongkou District. Results After the implementation of Regulations, administrative punishments for violators were more strictly. Meanwhile proprietors in public places fulfilled the added specific hygienic management requirements of the Regulations conscientiously. Moreover, the legal awareness was enhanced gradually. Conclusion The proprietors and employee in public places lack a basic knowledge of the Regulations. Therefore, it is recommended that more intensive efforts should be made to study, publicize and implement the Regulations through various channels. Legal awareness and supervise consciousness of the proprietors and employee should be intensified, respectively. Meanwhile, their own management should be normalized. Furthermore, the professional quality, the abilities of administration by law and the level of supervision and law enforcement of health supervisors should be improved, respectively. Thus the enduring hygienic management system of the public places will be established.