Abstract: Objective To learn the present application situation of Standards for blood storage and Technical specification for clinical blood transfusion during blood safety supervision at blood collecting agencies and medical institutions in Shanghai, and to provide a reference for strengthening blood storage safety supervision. Methods Questionnaires and retrospective study on blood storage inspection status were carried out to nine blood banks and 301 medical institutions in Shanghai from 2008 to 2015. Results Health administrative department carried out 2 574 times of blood safety supervision and inspection, and made a total of 12 blood safety administrative punishment from 2008 to 2015, the punishment rate was 0.47%. The tendency of applying Technical specification for clinical blood transfusion was found in health administrative departments and health supervision institutes regarding to administrative punishment, blood safety supervision and regulatory. Conclusion the priority and scope of application of Standards for blood storage and Technical specification for clinical blood transfusion should be cleared, and the standards should be improved.