Abstract: Objective To investigate the distribution of pathogens of neonate navel infection and their drug sensitivity to antibiotics,and to provide basis to choose rational antibiotics for clinical treatment of neonatalomphalitis.Methods Bacteria culture and antibiotic sensitivity test were undertaken for infected navel secretion of 114 newborn cases selected from Jan 2007 to Sep 2011 were conducted, the category and antibiotic sensitivity of the obtained 92 pathogens were analyzed.Results Among the 114 cases, 92 pathogens were isolated. positive strains 80.7%(92/114).The main pathogens were Gram-positive cocci accounted for 65.2%(60/92),the most of which were S.aureus 42.4%(39/92),coapulase-negative staphylococcus 22.8%(21/92).The Gram-negative bacilli pathogens took up 33.7%(31/92),among which Escherichia coli accounted for (9.8%)(9/92).K.Penumoniae accounted for (7.6%)(7/92),other Gram-negative bacilli pathogens 16.3%(15/92) and fungi 1.1%(1/92).MRS occupied 65.0%(39/60),E. coli and K.Penumoniae of ESBLs accounted for 55.6%(5/9) and 57.1%(4/7).The various bacteria were serous resistance to common antianicrobial agents.Conclusion The main pathogentic bacteria of the newborn omphlitis areS. aureus,S. epidermidis,S. Escherichia coli and K. pneumoniae.There is a trend of the increase of MRS and ESBLs pathogens.The various bacteria were multi-drug resistance to common antianicrobial agents.It is necessary to advise how attend to the newborn in order to decrease the newborn omphlitis.