Abstract: Objective To investigate the awareness of healthy lifestyle content among residents in Shanghai and to provide basis for future efforts. Methods Stratified random sampling covered all 18 districts in Shanghai. A total of 8548 participants were recruited and questionnaire with them was completed by face to face interview. Results Among Shanghai residents aged 18-69 years, 67.8% of them had awareness of all five items of healthy lifestyle content. And 94.6%, 92.3%, 80.7%, 77.3%, and 81.7% of them respectively had awareness of balanced diet, regular exercise, no smoking, no alcohol abuse, and keeping mental balance. Analysis of Logistic regression showed that gender, age, area of residence, and education level were factors influencing awareness rates mentioned above. Conclusion The information about healthy lifestyle content was effectively spread by various departments concerned and the Shanghai government. And the above results suggest that specific information spread for different demographic characteristics of different populations is beneficial for future improvement of healthy knowledge among them.